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renilla luciferase expression construct (prlnull vector  (Promega)

 
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    Structured Review

    Promega renilla luciferase expression construct (prlnull vector
    Renilla Luciferase Expression Construct (Prlnull Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/renilla+luciferase+vector+prlnull/pgl3+basic/pm29169731-50-0-9
    Average 90 stars, based on 1 article reviews
    renilla luciferase expression construct (prlnull vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Control:

    Article Title: Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3.
    Article Snippet: .. The internal control vector pRL-A3 was constructed by insertion of the PCR fragment of the A3 gene promoter (−128/+28) between the EcoRI and XhoI sites of the Renilla luciferase vector pRLnull (Promega) (Nishita and Takiya 2009). .. Gene transfection and luciferase activity assay The expression plasmids and reporter plasmids were introduced together into silk glands from day 1 fifth instar larvae with a gene gun (BioRad) according to Takiya et al. (2011) with several modifications.

    Plasmid Preparation:

    Article Title: Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3.
    Article Snippet: .. The internal control vector pRL-A3 was constructed by insertion of the PCR fragment of the A3 gene promoter (−128/+28) between the EcoRI and XhoI sites of the Renilla luciferase vector pRLnull (Promega) (Nishita and Takiya 2009). .. Gene transfection and luciferase activity assay The expression plasmids and reporter plasmids were introduced together into silk glands from day 1 fifth instar larvae with a gene gun (BioRad) according to Takiya et al. (2011) with several modifications.

    Article Title: The Xenopus Brachyury promoter is activated by FGF and low concentrations ofactivinandsuppressed by high concentrationsof activin and by paired-type homeodomain proteins
    Article Snippet: .. A −381/+54 fragment was transferred into the Renilla luciferase vector pRLNull (Promega) to generate the plasmid used in Figure A. Antennapedia and Bicoid sites in −381. .. Xbra2 were mutated using the Chameleon kit (Stratagene) and oligonucleotide 5′-GACTTGCAATTGATTCCCAGGATCCTCCATAGAGCT-3′.

    Construct:

    Article Title: Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3.
    Article Snippet: .. The internal control vector pRL-A3 was constructed by insertion of the PCR fragment of the A3 gene promoter (−128/+28) between the EcoRI and XhoI sites of the Renilla luciferase vector pRLnull (Promega) (Nishita and Takiya 2009). .. Gene transfection and luciferase activity assay The expression plasmids and reporter plasmids were introduced together into silk glands from day 1 fifth instar larvae with a gene gun (BioRad) according to Takiya et al. (2011) with several modifications.

    Polymerase Chain Reaction:

    Article Title: Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3.
    Article Snippet: .. The internal control vector pRL-A3 was constructed by insertion of the PCR fragment of the A3 gene promoter (−128/+28) between the EcoRI and XhoI sites of the Renilla luciferase vector pRLnull (Promega) (Nishita and Takiya 2009). .. Gene transfection and luciferase activity assay The expression plasmids and reporter plasmids were introduced together into silk glands from day 1 fifth instar larvae with a gene gun (BioRad) according to Takiya et al. (2011) with several modifications.

    Luciferase:

    Article Title: Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3.
    Article Snippet: .. The internal control vector pRL-A3 was constructed by insertion of the PCR fragment of the A3 gene promoter (−128/+28) between the EcoRI and XhoI sites of the Renilla luciferase vector pRLnull (Promega) (Nishita and Takiya 2009). .. Gene transfection and luciferase activity assay The expression plasmids and reporter plasmids were introduced together into silk glands from day 1 fifth instar larvae with a gene gun (BioRad) according to Takiya et al. (2011) with several modifications.

    Article Title: The Xenopus Brachyury promoter is activated by FGF and low concentrations ofactivinandsuppressed by high concentrationsof activin and by paired-type homeodomain proteins
    Article Snippet: .. A −381/+54 fragment was transferred into the Renilla luciferase vector pRLNull (Promega) to generate the plasmid used in Figure A. Antennapedia and Bicoid sites in −381. .. Xbra2 were mutated using the Chameleon kit (Stratagene) and oligonucleotide 5′-GACTTGCAATTGATTCCCAGGATCCTCCATAGAGCT-3′.



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    Promega renilla luciferase expression construct (prlnull vector
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    https://www.bioz.com/product/renilla+luciferase+vector+prlnull/pgl3+basic/pm29169731-50-0-9
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